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- Clone
- MC-480 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1
- Isotype
- Mouse IgM, κ
- Barcode Sequence
- GCTAGTTTGTGCTGC
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
125623 | 10 µg | 296⬠|
Description
The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.
Product Details
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeqâ¢-Bn oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
- SB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining in formalin-fixed, paraffin-embedded (FFPE) lymphoid tissue, and the oligomer sequence is confirmed by sequencing. TotalSeqâ¢-Bn antibodies are compatible with the 10x Visium CytAssist Gene and Protein Expression Assay.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution at 14,000xg at 2 â 8°C for 10 minutes before use. Carefully pipette out the liquid avoiding the bottom of the tube when handling. To determine and optimize dilutions for the addition of Totalseqâ¢-Bn antibodies into pre-designed antibody panels, refer to 10x Genomics Custom Add-on Antibody Optimization guide.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq⢠products. For example, for any technology platform Buyer uses with TotalSeqâ¢, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq⢠with that platform.- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
- Additional Product Notes
TotalSeq™-Bn reagents are designed to profile protein levels following an optimized protocol in spatial transcriptomics. Compatible spatial biology devices (e.g. Imaging System, 10x Genomics Visium Spatial CytAssist Gene and Protein Expression instruments and reagents) and sequencer (e.g. Illumina analyzers) are required. TotalSeq™-B reagents are not compatible with the 10x Genomics Visium system. The complete barcode sequence may be provided upon request. Please contact technical support for more information, or visit TotalSeq™-Bn Reagents for 10x Genomics Visium CytAssist Gene and Protein Assay.
- Application References
(PubMed link indicates BioLegend citation)
-
- Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565. (IHC, IP, WB)
Antigen Details
- Structure
- Carbohydrate epitope, Lewis blood group antigens
- Distribution
-
Murine embryonic stem cells (ES), embryonal carcinoma cells, 8-cell to blastocyst embryos; human differentiated cells, not on undifferentiated ES cells
- Function
- Adhesion, differentiation
- Cell Type
- Embryonic Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565.
2. Harris JF, et al. 1984. J. Immunol. 132:2502.
3. Gooi HC, et al. 1981. Nature 292:156.
4. Cui L, et al. 2004. J. Histochem. Cytochem. 52:1447. - Gene ID
- 14345 View all products for this Gene ID 2526 View all products for this Gene ID
- UniProt
- View information about CD15 on UniProt.org
Documentation
- Technical data sheet
- Certificate of Analysis
- Safety Data Sheet
Reviews
Review This Product
Related Products
Description | Clone | Applications |
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Pathways
- Innate Immunity
- Stem Cells and Development
Pages
- Quality Control
- Multicolor Staining Guide
- Fluorescence Spectra Analyzer
- CD Molecule Chart
- Fluorophore Brightness Index
- Custom Panel Construction
- Fluorophore Equivalency Tool
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
-
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
-
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
-
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
-
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining Iâm seeing in my tissue is real?
-
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All CD15 Reagents Request Custom Conjugation
Description | Clone | Applications |
---|---|---|
PE anti-mouse/human CD15 (SSEA-1) | MC-480 | FC |
Biotin anti-mouse/human CD15 (SSEA-1) | MC-480 | FC |
Purified anti-mouse/human CD15 (SSEA-1) | MC-480 | FC,IP,WB,IHC-F,IHC-P |
Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) | MC-480 | FC |
Alexa Fluor® 488 anti-mouse/human CD15 (SSEA-1) | MC-480 | FC |
FITC anti-mouse/human CD15 (SSEA-1) | MC-480 | FC |
TotalSeq™-A0076 anti-mouse/human CD15 (SSEA-1) | MC-480 | PG |
APC anti-mouse/human CD15 (SSEA-1) | MC-480 | FC |
TotalSeq™-C0076 anti-mouse/human CD15 (SSEA-1) | MC-480 | PG |
TotalSeq™-B0076 anti-mouse/human CD15 (SSEA-1) | MC-480 | PG |
TotalSeq™-Bn0076 anti-mouse/human CD15 (SSEA-1) | MC-480 | SB |
Certificate of Analysis
For Research Use Only. Not for diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.
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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse/human CD15 (SSEA-1)
F9 (mouse embryonic carcinoma cell line) stained with MC-480... -
Biotin anti-mouse/human CD15 (SSEA-1)
F9 (mouse embryonic carcinoma cell line) stained with biotin... -
Purified anti-mouse/human CD15 (SSEA-1)
F9 (mouse embryonic carcinoma cell line) stained with purifi... Human peripheral blood lymphocytes, monocytes, granulocytes ... -
Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1)
F9 (mouse embryonic carcinoma cell line) stained with MC-480... -
Alexa Fluor® 488 anti-mouse/human CD15 (SSEA-1)
F9 (mouse embryonic carcinoma cell line) stained with MC-480... -
FITC anti-mouse/human CD15 (SSEA-1)
F9 cells (mouse embryonic carcinoma cell line) were stained ... -
TotalSeq™-A0076 anti-mouse/human CD15 (SSEA-1)
-
APC anti-mouse/human CD15 (SSEA-1)
Murine embryonic carcinoma cell line F9 was stained with CD1... -
TotalSeq™-C0076 anti-mouse/human CD15 (SSEA-1)
-
TotalSeq™-B0076 anti-mouse/human CD15 (SSEA-1)
-
TotalSeq™-Bn0076 anti-mouse/human CD15 (SSEA-1)